发明名称 |
DNA AMPLIFICATION AND SEQUENCING USING DNA MOLECULES GENERATED BY RANDOM FRAGMENTATION |
摘要 |
The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3' end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention. |
申请公布号 |
WO03050242(A3) |
申请公布日期 |
2003.10.23 |
申请号 |
WO2002US37322 |
申请日期 |
2002.11.13 |
申请人 |
RUBICON GENOMICS INC.;MAKAROV, VLADIMIR, L.;SLEPTSOVA, IRINA;KAMBEROV, EMMANUEL;BRUENING, ERIC |
发明人 |
MAKAROV, VLADIMIR, L.;SLEPTSOVA, IRINA;KAMBEROV, EMMANUEL;BRUENING, ERIC |
分类号 |
C12N15/09;C07H19/00;C07H21/02;C07H21/04;C12N;C12P19/34;C12Q1/48;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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