MODIFIED TISSUE PLASMINOGEN ACTIVATOR

摘要

A DNA construct containing a nucleotide sequence consisting essentially of a first region encoding a fibrin- binding domain and a second region positioned downstream of the first region, with the second region encoding a catalytic domain for the serine protease activity of tissue-type plasminogen activator is disclosed. The nucleotide sequence codes for a protein which has substantially the same biological activity as t-PA. The regions may be derived from genomic clones or cDNA clones of t-PA, or may be constructed by conventional DNA synthesis techniques. Expression vectors capable of directing the expression of the t-PA-like protein, and transfected or transformed cells producing such a protein, are also disclosed.