| 摘要 |
A novel process for extraction, isolation and separation of taxanes, particu larly paclitaxel, from natural sources such as bark, nee dles and twigs from Taxus species, tissue cultures, and fungi is described, where in the taxanes are separated from the crude extracts by partitioning between polar and nonpolar solvents, precipitation in nonpolar solutions, reacting the mixture by halogenation of unsat urated taxane derivatives, followed by chromatographically separating and crystalli zing taxanes from a mixture of polar and nonpolar solven ts; more particularly, the process of halogenation of unsaturated side chain tax ane derivatives, particularly cephalomannine in presence of paclitaxel, is described, wherein bromine is preferably added to the double bond of unsaturated taxanes leaving paclitaxel unchanged , with paclitaxel being easily separated from the mixture including the less polar halogenated taxane derivatives.
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