发明名称 |
NUTRIENT MEDIUM FOR CULTURE OF ANIMAL CELL (VARIANTS) AND METHOD FOR PREPARING PROTEIN USING MEDIA |
摘要 |
FIELD: biotechnology. SUBSTANCE: invention proposes nutrient media for culture of animal cell containing fish meat extract or product of enzymatic decomposition of fish meat instead components preparing from mammals. Also, invention proposes method for preparing protein by culturing animal cell in such medium. The proposed nutrient medium allows stable culturing animal cells without using expensive protein that is varied by quality significantly, such as bovine fetal serum and allows to exclude risk of pollution with anomalous prions or viruses and to obtain safety technological products. The proposed method for preparing protein allows to simplify isolation and purification of desirable protein produced by animal cell from nutrient medium. EFFECT: improved preparing method, valuable properties of medium. 12 cl, 9 dwg, 5 ex
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申请公布号 |
RU2214455(C2) |
申请公布日期 |
2003.10.20 |
申请号 |
RU20000133243 |
申请日期 |
1999.06.01 |
申请人 |
CHUGAI SEJJAKU KABUSIKI KAJSJA |
发明人 |
SIBUJA KAZUSI;ATSUMI MASARU;TSUNAKAVA SIGEJUKI;NOGAKI KANEO |
分类号 |
C12N5/00;C12N5/02;C12N5/07;C12N5/16;C12P21/00;C12P21/04;(IPC1-7):C12N5/06 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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