| 摘要 |
PURPOSE:To evaluate easily and rapidly the method of immobilization of physiologically active organism, by measuring potential change between electrode for evaluation of immobilization method and having an ion microsensor inserted into the immobilized physiologically active organism, and a reference electrode. CONSTITUTION:Electrodes 5, 6 for evaluation of immobilization method of physiologically active organism and a small-sized reference electrode 7 such as an Ag-AgCl electrode, etc. are immersed in liquid substrate 3 in a vessel 4. The control of these electrodes, and the detection of signals from the electrodes 5, 6 is carried out through an amplifier 9, and recorded separately on a recorder 10. When both immobilization methods by agar gel and polyacrlylamide gel are evaluated by using, for example, lactic acid bacteria as microorganism, the former method is provided better than the latter with respect to substrate and diffusion of organic acids, and to less inactivation of lactic acid bacteria accompanying immobilization. |
