Methods of producing a library and methods of selecting polynucleotides of interest

摘要

The present invention relates to a high efficiency method of introducing DNA into linear DNA viruses such as poxvirus, a method of producing libraries in linear DNA viruses such as poxvirus, and methods of selecting or screening for polynucleotides of interest based on cell nonviability or other phenotypes of eukaryotic cells, especially mammalian cells.