| 摘要 |
Disclosed herein is a reversible carbohydrate (glycan) trap, methods and system for preparing the trap and use of the trap in preparing and trapping glycans for the study of carbohydrate structure. The device and methods assist in the structural characterization of glycans. The method traps and purifies carbohydrate structures at improved sensitivities, thereby enhancing the accuracy of a tissue or cell's complete carbohydrate composition, the glycome, (vis-a-vis proteome). Most basically, the trap device covalently binds activated glycosyl residues on a polymer specifically prepared to contain aldehydic funactional groups. The trap material is formed from a carbohydrate polymer, preferably dextran, which is oxidized with periodate to introduce aldegydic groups. The oxidized dextran polymer is packable into separation columns or micro-tips for use as the glycan trap. Glycans treated with hydrazine, to form glycosyl hydrazyls, may then be added to the oxidized carbohydrate polymer, (ODP), trap wherein they are covalently bound to the aldehyde groups of the trap. To release the bound glycosyl hydrazyls, anhydrous hydrazine may be added at approximately room temperatures, thereby releasing the glycans as glycosylhydrazyls which may then be studied further.
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