摘要 |
<p>Apparatus and method for acquiring an infrared spectrum of a sample is described. In the apparatus a solublized sample suspected of having materials with an infrared spectrum including an amide I band, an amide II band, an amide III band, an amide A band, an OH stretching region or a combination thereof is brought into contact with the functionalized tip of an internal reflecting element (IRE) in a sample cell to form a sample-IRE interface. A beam of infrared radiation is directed from a source through the IRE under conditions that it interacts with the sample-IRE interface once. The reflectane profile of the radiation is detected and recorded over a range of frequencies to obtain an infrared spectrum of the sample. The infrared spectra can be obtained and stored in a database to form a library.</p> |