| 摘要 |
It is needed to implement probing sequences having no potentiality of cross-hybridization, equal Tms, and less secondary structures for the probing sequences for DNA arrays so that the sensitivity and repeatability of measurement may be ensured. The probing sequences for the DNA arrays can be determined by following at least two steps, one being a step for creating a list of candidate probes for the DNA probe arrays, which are made of partial sequences of genome or cDNA sequences (the list-up step) and the other being a step for discarding the candidate probes, of which melting temperatures deviate from a given range, which have higher stability of secondary structures, or which have the potentiality of cross-hybridization, from the primary candidate probe list (the filtering step). The present invention thus provides a method for designing probing DNA sequences to be fixed on the DNA probe arrays.
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