PHAGE DISPLAY AFFINITY AND SPECIFICITY FILTERS AND FORWARD SCREEN
摘要
The invention provides an affinity filter for the binding of phage-displayed proteins to dissolved target molecules. The phage-displayed proteins are contacted with immobilized target in the presence and absence of dissolved target; the behavior of the phage-displayed proteins as a function of concentration of dissolved target permits approximation of the affinity of the phage-displayed protein for target. The invention also provides a method to screen large numbers of compounds for their ability to complete with a compound known to bind a phage-displayed protein.
申请公布号
WO03084981(A2)
申请公布日期
2003.10.16
申请号
WO2003US10247
申请日期
2003.04.02
申请人
AMBIT BIOSCIENCES CORPORATION
发明人
LOCKHART, DAVID, J.;TREIBER, DANIEL, KELLY;ZARRINKAR, PATRICK, P.