A method is provided for separating a protein from one or more other proteins using hydroxyapatite chromatography in which the protein does not bind to hydroxyapatite but the other protein(s) does. In some embodiments, a second protein affixed to a solid support has been used previously to purify the protein by affinity chromatography, and small amounts of the second protein are introduced in the sample during this process. The protein being purified can comprise at least one constant antibody immunoglobulin domain. The second protein can bind to proteins comprising such a domain.
申请公布号
WO03059935(A3)
申请公布日期
2003.10.16
申请号
WO2002US41159
申请日期
2002.12.20
申请人
IMMUNEX CORPORATION;VEDANTHAM, GANESH;BROOKS, CLAYTON, A., III;REEDER, JOANNE, M.;GOETZE, ANDREW, M.
发明人
VEDANTHAM, GANESH;BROOKS, CLAYTON, A., III;REEDER, JOANNE, M.;GOETZE, ANDREW, M.