发明名称 |
GENE DISRUPTED YEAST |
摘要 |
The invention provides a yeast in which a specific gene alone has been disrupted and to which auxotrophy is impared to construct a system for producing an industrially useful substance through gene recombination by utilizing characteristics of a yeast. <??>In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is transformed by a gene expression cassette containing a biodegradable polyester synthase gene and the obtained transformant is cultured to thereby produce biodegradable polyester. |
申请公布号 |
EP1352958(A1) |
申请公布日期 |
2003.10.15 |
申请号 |
EP20020716318 |
申请日期 |
2002.01.18 |
申请人 |
KANEKA CORPORATION |
发明人 |
FUKUCHI, TAKESHI;YOKOMIZO, SATORU;OSAKADA, FUMIO;MATSUMOTO, KEIJI;TAKAGI, MASAMICHI;OTA, AKINORI |
分类号 |
C12N15/09;C07K14/39;C12N1/19;C12N9/00;C12N15/90;C12P7/62;C12R1/72 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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