发明名称 |
Rapid method of detection and enumeration of sulfide-producing bacteria in food products |
摘要 |
A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. A plurality of fluorescence measurements are taken during an incubation period of about 4 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. A visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective in about 4 hours to 17 hours at 30° C.</PTEXT>
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申请公布号 |
US6632632(B1) |
申请公布日期 |
2003.10.14 |
申请号 |
US20010016854 |
申请日期 |
2001.12.14 |
申请人 |
COLIFAST AS |
发明人 |
LORENTZEN GRETE;SKJERDAL OLAUG TARAN;BERG JAMES D. |
分类号 |
C12Q1/04;C12Q1/06;C12Q1/08;(IPC1-7):C12Q1/04;C12Q1/00;C12Q1/22;C12Q1/02 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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