SYSTEM AND METHOD FOR QUANTITATING TRANSCRIPTION FACTORS

摘要

Methods for detecting the presence of at least one, usually a plurality of, DNA binding proteins, e.g., transcription factors, in a sample, both qualitatively and quantitatively, are provided. In the subject methods, a substrate having one or more DNA probes immobilized on a surface thereof, one for each DNA binding protein of interest, is contacted with a sample under conditions sufficient for binding complexes of the probes and their respective DNA binding proteins to be produced. The sample may be purified with respect to one or more DNA binding proteins or be a cellular/nuclear extract. Resultant binding complexes on the surface of the substrate are then detected and related to the presence of the DNA binding protein-DAN interations of interest in the sample. Also provided are devices and systems for use in practicing the subject methods. The subject methods find use in a variety of different applications, e.g., detecting the presence of a transcription factor in a sample, the study of transcription factor profiles in response to a given stimulus, screening for therapeutic agents, and the like.