METHODS FOR PRODUCING SECRETED POLYPEPTIDES HAVING L-ASPARAGINASE ACTIVITY

摘要

The present invention relates to recombinant methods for producing a secreted polypeptide having L-asparaginase activity, comprising (a) cultivating under conditions conducive for production of the polypeptide a host cell comprising a nucleic acid construct comprising a first nucleic aci d sequence encoding a secretory signal peptide operably linked to second nucle ic acid sequence encoding the polypeptide having L-asparaginase activity, where in the signal peptide directs the polypeptide into the cell's secretory pathway ; and (b) recovering the secreted polypeptide. The present invention also relates to isolated polypeptides having L-asparaginase activity and nucleic acids thereof.