LAMBDA INTEGRASE MEDIATED RECOMBINATION IN PLANTS

摘要

The present disclosure provides methods for obtaining the targeted integrati on of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DN A within a plant cell, comprising : introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site ; introducing in to the plant cell a donor nucleotide sequence comprising a second Int recogniti on site ; and introducing into the plant cell an integrase or integrase complex .