| 摘要 |
A method of identifying a compound which modulates the interaction between protein kinase B (PKB) and phosphatidylinositol (3,4,5)-triphosphate (PtdIns(3,4,5)P3) or phosphatidylinositol (3,4)-diphosphate (PtdIns(3,4)P2), the method comprising determining whether, and optionally to what extent, a conformational change in a pleckstrin homology (PH) domain of PKB upon contact with PtdIns(3,4,5)P3 or PtdIns(3,4)P2, is enhanced or disrupted in the presence of the compound. A method of identifying a compound which mimics the effect of PtdIns(3,4,5)P3 or PtdIns(3,4)P2 on PKB, the method comprising determining whether, and optionally to what extent, a conformational change in a PH domain of PKB upon contact with the compound, matches a conformational change in the PH domain upon contact with the PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
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