DECOY COMPOSITIONS FOR TREATING AND PREVENTING BRAIN DISEASES AND DISORDERS

摘要

It is intended to transfection of an NF-kappaB decoy oligodeoxynucleotide into rat cranial nerve via the carotid artery under global brain ischemia. As the results of polymerase chain reaction, it is found out that the transfected NF-kappaB decoy oligodeoxynucleotide effectively inhibits the expression of tumor necrosis factor alpha, interleukin 1beta and intracellular adhesion molecule 1 messenger RNA within 1 hour after the global brain ischemia. As the results of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end label staining method and microtube-binding protein 2 immunohistochemistry, it is found out that the transfected NF-kappaB decoy oligodeoxynucleotide significantly relieves nerve injury within 7 days after global brain ischemia. Therapeutic transfection with the NF-kappaB decoy oligodeoxynucleotide during brain ischemia is efficacious in relieving nerve injury, which suggests a strategy for protecting the brain from global ischemia.