| 摘要 |
PURPOSE: A peptide gene carrier and a method for transfecting a gene into a cell using the same are provided, thereby stably transfecting the gene into the cell without cell toxicity. CONSTITUTION: A peptide gene carrier comprises a plurality of arginine residues, wherein the number of arginine is 5 to 14, preferably 12. A method for transfecting a gene into a cell using the peptide gene carrier comprises the steps of: (a) preparing a mixture of peptide gene carrier comprising a plurality of arginine residues, polynucleotide to be transfected, and a binding agent capable of binding the peptide gene carrier with the polynucleotide; and (b) applying the mixture to the cell, wherein the binding agent is a buffer solution having the pKa value of 6.5 to 8.5; the binding agent is phosphate buffer solution, serum free cell cultured medium, HEPES buffer solution, and MOPS buffer solution; and the cell is either normal animal cell or tumor animal cell. |
