Array based hybridization assays employing enzymatically generated labeled target nucleic acids and compositions for practicing the same

摘要

Methods for assaying a sample for the presence of one or more nucleic acid analytes, either qualitatively or quantitatively, are provided. In the subject methods, an array of DNA primers is contacted with the sample being assayed under conditions sufficient to produce labeled target nucleic acids enzymaticallyon the surface of the array at locations where a DNA primer has hybridized to a nucleic acid analyte to produce a duplex nucleic acid. The presence of labeled target nucleic acids on the array surface is then detected and used to determine the presence of the one or more nucleic acid analytes in the sample. Also provided are kits for practicing the subject methods. The subject invention finds use in a variety of different applications, including differential gene expression analysis.