| 摘要 |
The invention relates to a method for producing a random arrangement of clonal islands on a surface, comprising the following steps a1) preparing a surface having at least one priming type whose primer molecules are immobilised in an at least partially reversible manner on the surface; b1) hybridising the nucleic acids which need to amplified with the primers from step a1); c1) amplification of the nucleic acids from step b1) which need to be amplified, at least one counter primer type is being used for amplification, the primer molecules thereof being at least partially non immobilised and whose primer molecules containing the primer from step a1) form at least one PCR- primer pair with respect to the nucleic acids which are to be amplified. The reaction area of the amplification is formed by the area which is defined by the surface and by a micro-compartmenting matrix adjacent to the surface. |
