发明名称 |
Regulation of HIV-Tat and Nef by PAK4 kinase and its binding partners and methods of identifying modulators thereof |
摘要 |
The present invention discloses complexes of cellular signaling proteins that interact in vivo with the HIV-encoded auxiliary proteins Nef and Tat to modulate their activity. This complex includes the novel serine/threonine kinase PAK4 and the novel guanine nucleotide exchange factor Cdc42-GEF, which synergize to stimulate Tat transcriptional activity, and the acetyl-transferase Tip60 which modifies Nef. These cellular partners of the HIV auxiliary proteins represent novel targets for HIV therapeutics. The invention provides isolated DNA and vectors encoding PAK4 and Cdc42-GEF, and methods of producing recombinant forms of these proteins. The invention also provides methods for identifying compounds that modulate the activity of HIV-Tat, HIV-Nef or Tip60, and methods for modulating the activity of these enzymes.
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申请公布号 |
US2003186254(A1) |
申请公布日期 |
2003.10.02 |
申请号 |
US20020134102 |
申请日期 |
2002.04.29 |
申请人 |
CELL SIGNALING TECHNOLOGY, INC. |
发明人 |
MELNICK MICHAEL B.;MORITZ ALBRECHT;COMB MICHAEL J. |
分类号 |
C07K14/47;C12N7/01;C12N9/12;(IPC1-7):C12Q1/70;C12Q1/68;C12P19/34;C12N7/00;C12N5/06;C12N5/16 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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