MODIFIED OLIGOMER OF NUCLEIC ACID, METHODS FOR ITS PREPARING, MODIFIED CONDUCTING SURFACES, METHODS FOR THEIR PREPARING, METHOD OF ELECTROCHEMICAL ASSAY OF EVENTS OF HYBRIDIZATION OF NUCLEIC ACID OLIGOMERS

摘要

FIELD: molecular biology, biochemistry. SUBSTANCE: invention relates to method of electrochemical assay of events of sequence-specific hybridization of oligomers of nucleic acid. For this purpose, single-stranded oligomeric DNA, RNA or PNA are bound by their end with surface of a carrier and by another free end they are bound covalently with oxidative-reducing pair and used as hybridizing matrix (probe). As result of treatment with solution of oligonucleotide (target) to be studied the electric bind between conducting surface of carrier and oxidative- reducing pair that are bound by a single-stranded oligonucleotide being the latter is absent or weak initially is subjected for modification. In the case of hybridization this electric bind between surface of carrier and oxidative-reducing pair that are bound now by hybridized single-stranded oligonucleotide is enhanced. This allows to assay events of hybridization using methods of cyclic voltammetry, amperometry or measurement of conductivity. EFFECT: improved preparing method. 51 cl, 9 ex