Serological diagnosis of protostrongylidae infections and identification of unique antigens

摘要

An isolated immunogenic Protostrongylidae antigen selected from the group consisting of a P. tenuis-specific 20 kDa antigen, a P. tenuis-specific 37 kDa antigen, an E.Cervi-specific 37 kDa antigen, an E.Cervi-specific 52 kDa antigen, a P. tenuis-specific 75 kDa antigen, a common Protostrongylidae 105 kDa antigen and a common Protostrongylidae 158 kDa antigen, as determined by SDS- PAGE gel electrophoresis. Also disclosed are the use of such antigens for diagnosing Protostrongylidae infection in a vertebrate subject; isolated antibodies that recognize an epitope on the antigen; the use of an excretory-secretory products of the larvae of the protostrongylid, ES-L3 antigen or sL3 antigen, in the manufacture of a composition for detecting the presence of Protostrongylidae infection in a biological sample.