发明名称 |
A RAPID AND SENSITIVE PROXIMITY-BASED ASSAY FOR THE DETECTION AND QUANTIFICATION OF DNA BINDING PROTEINS |
摘要 |
Methods to determine the activity of any and all DNA binding factors, proteins or fragments thereof based upon the detection of a change in a luminescence or fluorescence signal are provided. Preferably, a fluorescence donor is attached to a nucleic acid comprising one portion of a DNA binding element and a fluorescence acceptor is attached to a nucleic acid comprising the other portion of the same binding element. Alternatively, a microsphere bead is attached to a nucleic acid comprising one portion of a binding element and a luminescent moiety or fluorochrome is attached to a nucleic acid comprising the other portion of the same binding element. Binding of a DNA binding factor to the nucleic acid components affects a change in luminescence. These methods may also be used to detect mediating analytes, to diagnose diseases and/or screen for drugs that mediate the activity of DNA binding factors. |
申请公布号 |
WO03078449(A2) |
申请公布日期 |
2003.09.25 |
申请号 |
WO2002US24822 |
申请日期 |
2002.08.02 |
申请人 |
HEYDUK, TOMASZ;SAINT LOUIS UNIVERSITY |
发明人 |
HEYDUK, TOMASZ |
分类号 |
G01N21/64;C12M1/00;C12M1/34;C12N15/09;C12Q1/68;G01N21/76;G01N33/53;G01N33/542;G01N33/566;G01N33/58;G01N33/68 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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