摘要 |
<p>Compositions and methods for identifying imprinting and genes regulated by imprinting are provided. The methods involve an analysis of the nucleotide sequence and the identification of CpG islands. At least two islands are involved in imprinting. Thus, genes can be identified that are differentially expressed based on parental inheritance. In this manner, the methods are useful for determining the propensity of a gene to be influenced by imprinting. Such analysis involves determining the pattern of imprinting for cells of interest. Fig. 2A shows the pattern of paternal and maternal ZmFie1 allele expression in developing kernels. It is further recognized that DNA constructs can be constructed which show differential expression depending upon the parent-of-origin. To silence a paternally inherited allele, at least two CpG islands are utilized in the construct.</p> |