| 摘要 |
This invention concerns a method for the lysis of prokaryotic or eukaryotic cells, or for simultaneous lysis of both prokaryotic and eukaryotic cells It consists in combining at least three of the following parameters: a mass of active, small-diameter beads corresponding to 50% or less than the mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of between 10 and 20 minutes, and/or seven (7) or fewer non-lysing glass beads to drive the movement of the lysing beads, and/or between five (5) and fifteen (15) non-lysing iron beads to drive the movement of the lysing beads, depending on the technique being used, i.e. either sonication, mechanical vortex centrifugation or magnetic vortex centrifugation. The invention is particularly applicable in the field of cell lysis.
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