PROTEIN CAPABLE ON INHIBITING COLLAGEN STIMULATED AGGREGATION OF THROMBOCYTES AND PREVENTING INTERACTION BETWEEN COLLAGEN AND vWF, METHOD OF PREPARING THEREOF, PHARMACOLOGICAL COMPOSITION BASED THEREON

摘要

biotechnology and medicine. SUBSTANCE: protein (Brandinin) has molecular weight of about 15kDa and binds with collagen. Protein inhibits binding of collagen with THROMBOCYTES and subsequent activation thereof which causes aggregation of THROMBOCYTES and formation of thrombuses. Protein prevents binding of von Villebrand factor with collagen. Protein is isolated from crude extracts of leech Hirudo medicinalis by electrophoresis in polyacrylamide gel. Protein is useful for prophylaxis and treatment of thrombolytic diseases. EFFECT: improved properties of protein. 5 cl, 5 dwg, 6 ex, 2 tbl