DNA SEQUENCES OF MAJOR SECRETED PROTEINS FROM THE CILIATE TETRAHYMENA AND USE THEREOF

摘要

A regulatory element of a DNA for an efficient heterologous expression of proteins in Tetrahymena ssp which efficient heterologous expression is performed under control of promotors and/or terminators which are derived from in Tetrahymena ssp naturally occurring DNA comprising promotors and/or terminators and a coding region for proteins secreted ion a high level and the expression of proteins secreted on a high level is independent of the cell-cycle of Tetrahymena ssp.Furthermore a method is disclosed for the heterologous expression of proteins from Tetrahymena using gene constructs made from regulatory elements selected from the group consisting of promoters or terminators from Tetrahymena and coding nucleic acid sequences of a protein to be expressed heterologously, said regulatory elements from Tetrahymena being obtainable by: two-dimensional gel electrophoretical separation and isolation of the proteins (CMSP) selected from the group according to table 1; determination of at least one partial amino acid sequence of the proteins; establishing the nucleic acid sequence of the proteins and therefrom establishing the gene which codes for these proteins; establishing the regulatory elements of the coding region of said proteins.