| 摘要 |
The invention relates to a method for illuminating particles contained in a medium for optical analysis, and an optical particle analyser. The medium is in contact with a window and subjected to a light beam that comes from a light source, through the window, and illuminates the particles to form an image of the particles; the window that is used being a trapezoidal prism a longer side surface of the parallel side surfaces of which forms a surface facing the medium. The light beam is modified to provide a beam having a predetermined thickness in the direction the window normal, the light beam being directed to pass through one of the oblique surfaces of the prism serving as the window and to further travel inside the medium containing particles in the vicinity of the window surface making contact with the medium, substantially parallel to the surface, to thereby illuminate the particles contained in a precisely predefined volume portion of the medium, the image being formed by the light reflected back from the particles through the window. In the vicinity of the prism serving as the window the light beam coming from the light source is directed to pass through a mirror surface and a cylindrical lens, which direct the light beam to the prism and convert a shift of the light source to a change in the light beam angle.
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