| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for analyzing flora, having ability of corresponding to a clinical site and evaluation of environmental effects because of short analyzing time in the flora analysis, having excellent repeatability of an analysis result of a gene-extracted specimen, hardly requiring specialty, easily analyzable, and contributing to the development in a microbial ecology field and a bio- industry field as a method by which the species of a bacterium is estimated in high selectivity in the flora analysis. SOLUTION: A PCR is carried out by using a chromosomal DNA derived from a bacterium by using a labeled primer set capable of amplifying a region corresponding to the base number 500 or later of Escherichia coli 16S rRNA gene (GenBank accession number V00348) as a template, and T-RFLP analysis based on the digestion by a restriction enzyme BfaI, RsaI, TagI, HhaI or BslI, or a restriction enzyme recognizing the same base sequence as they recognize is carried out. The fragment obtained by the T-RFLP is cloned directly or after amplification by a PCR by Escherichia coli or the like, and the base sequence is determined. COPYRIGHT: (C)2003,JPO
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