CELL BASED POSITIVE ASSAY SYSTEM USING REPRESSOR OF HDAC

摘要

PURPOSE: Provided is a cell based assay system which inhibits the expression of a reporter by the activation of a repressor, and induces the expression of the reporter upon receipt of proper signals, thereby evaluating the given signals. CONSTITUTION: A specific inhibition regulating gene and a constant expression gene are constructed before the target gene such that the target gene is constantly expressed under the normal circumstances, but specifically repressed by a specific repressor. The activity of histone deacetylase(HDAC) is operated as the specific repressor. The activity of the HDAC within the cell is traced using a vector with the HDAC fused at the DNA binding domain.