发明名称 |
TAGGING AND RECOVERY OF ELEMENTS ASSOCIATED WITH TARGET MOLECULES |
摘要 |
The invention provides a method for identifying elements associated with a target molecule comprising the steps of: (a) providing a probe capable of binding by specific molecular interaction to a predetermined specifically defined region of a target molecule, the probe associated with or capable of recruiting an enzyme; (b) adding a tag capable of being activated by the enzyme such that it can attach to elements in the vicinity of the enzyme; an d (c) isolating elements having the tag attached thereto, wherein the defined region occurs once, twice, or in a low number of copies in the target molecule. Preferably the tag can attach only to elements in the vicinity of the enzyme. |
申请公布号 |
CA2481312(A1) |
申请公布日期 |
2003.09.18 |
申请号 |
CA20032481312 |
申请日期 |
2003.03.07 |
申请人 |
THE BABRAHAM INSTITUTE |
发明人 |
CARTER, DAVID;CHAKALOVA, LYUBOMIRA;FRASER, PETER |
分类号 |
G01N33/53;C12N15/09;C12Q1/28;C12Q1/68;G01N33/15;G01N33/50;G01N33/58;G01N33/68;(IPC1-7):G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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