The present invention provides the use of recombinantly produced serine proteases, such as bovine trypsin produced in corn, for the production and propagation of viruses in vitro cell cultures and discloses methods for the production of viral batches in serum-free tissue culture, without using animal-derived trypsin.
申请公布号
WO03076462(A1)
申请公布日期
2003.09.18
申请号
WO2002NL00157
申请日期
2002.03.08
申请人
CRUCELL HOLLAND B.V.;PAU, MARIA, GRAZIA;UYTDEHAAG, ALPHONSUS, GERARDUS, CORNELIS, MARIA
发明人
PAU, MARIA, GRAZIA;UYTDEHAAG, ALPHONSUS, GERARDUS, CORNELIS, MARIA