发明名称 |
Devices and methods for isolating and recovering target cells |
摘要 |
A cell isolating device and method is provided to concentrate or isolate cells with specific characteristics from a mixture of different cell types. One embodiment may comprise two subtypes of antibodies that are directly conjugated to biotin (Abb) and conjugated to a fluorescent molecule (Abf). The conjugated antibodies (Abb+Abf) bind to the target cells in a mixed cell suspension. The cell suspension is then passed over an immobilized avidin or streptavidin substrate on a glass microscope slide. The biotinylated target cells adhere to the avidin/streptavidin substrate, while the unbound cells are washed off and collected in a wicking member. Captured cells on the avidin/streptavidin substrate may then be visualized directly using a fluorescent microscope or detected and enumerated via an on-board fluorescent detection device. Additional chemicals and/or physical manipulation may then be applied to the device to release viable target cells for subsequent analysis.
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申请公布号 |
US2003175818(A1) |
申请公布日期 |
2003.09.18 |
申请号 |
US20020247223 |
申请日期 |
2002.09.18 |
申请人 |
ROSS AMELIA A.;BERNSTEIN STEVE |
发明人 |
ROSS AMELIA A.;BERNSTEIN STEVE |
分类号 |
C12M1/34;C12N5/00;C12N5/06;G01N33/483;G01N33/569;(IPC1-7):C12Q1/68;G01N33/53;G01N33/567 |
主分类号 |
C12M1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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