Detection of human immunodeficiency virus type 1

摘要

Amplification oligonucleotides and hybridization assay probes are provided for use in the amplification of the pol region of Human Immunodeficiency Virus type 1 (HIV-1) target nucleic acid for the detection of HIV-1 in a sample. More particularly, there is provided a first primer oligonucleotide sequence able to hybridize to a region of the nucleic acid of HIV corresponding to HIV-1 HXB2 bases 4756-4778, with a predicted Tm for the hybrid of said first primer to HIV nucleic acid of about 65 DEG C, and a second primer oligonucleotide sequence able to hybridize to a region of the nucleic acid of HIV corresponding to HIV-1 HXB2 bases 4835-4857, with a predicted Tm for the hybrid of said second primer to HIV nucleic acid of about 65 DEG C.