RECOMBINANT DNA AND EXPRESSION VECTOR

摘要

The recombinant DNA according to the present invention contains a regulator region which is at least 50 % homologous to the sections -969 to -991 base pairs and/or -1308 to -1330 base pairs of the sequence in Fig. and in the 3' direction from the regulation sequence contains at least one promoter region which in turn contains a -35/-10 promoter sequence. An expression vector according to the present invention contains a recombinant DNA according to the present invention ligated into a suitable vector molecule. To prepare the recombinant DNA according to the present invention, the pfl gene with its upstream regions is isolated from the gene bank of a microorganism, the desired parts are obtained according to well-known methods and they are combined with the other desired sequences or promoters. When using a recombinant DNA according to the present invention or an expression vector according to the present invention for the inducible and the repressible expression of a foreign gene, the induction is effected under anaerobic conditions and by pyruvate and the repression is effected by oxygen.