INTERLEUKIN-3 MULTIPLE MUTATION PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide a recombinant human interleukin-3 (hIL-3) variant or mutant protein (mutein).

SOLUTION: The hIL-3 mutein contains amino acid substitutions and may also have amino acid deletions at both the N- and the C-termini. A pharmaceutical composition comprises the hIL-3 mutein. A method for using the same is provided. Furthermore, a recombinant expression vector comprising a nucleotide sequence encoding the hIL-3 mutein, a related microbial expression system and a method for producing the hIL-3 mutein using the microbial expression system are provided. Deleted muteins of the hIL-3 in which 1-14 amino acids are deleted from the N-terminus, 1-15 amino acids (119-133) are deleted from the C-terminus, and in which the amino acid substitutions are contained in the polypeptide are provided. The hIL-3 multiple mutation polypeptide may have a biological activity similar to or better than that of the hIL-3 and may have an improved adverse effect profile.

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