Non-denatured, recombinant human immunodeficiency virus (HIV) Tat that is free of bacterial RNA and endotoxin is employed in an anti-HIV vaccine. A process of producing the recombinant Tat protein includes steps for removing bacterial RNA from the recombinant Tat and for removing endotoxin from the recombinant Tat protein. A Tat-adsorbed nanoparticle formulation and method of making the same. A method of vaccinating against and/or treating HIV infection comprises administering to a subject in need of such vaccination or treatment an immune­response inducing effective amount of the recombinant Tat protein.
申请公布号
WO03073984(A2)
申请公布日期
2003.09.12
申请号
WO2003US05806
申请日期
2003.02.27
申请人
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION;NATH, AVINDRA;MUMPER, RUSSELL, J.;WOODWARD, JEROLD, G.;CUI, ZHENGRONG