METHOD AND DEVICE FOR PARALLEL ANALYSIS OF BIO MOLECULES

摘要

The invention relates to a method and device for parallel separation of a plurality of individual samples (29) in a 3-dimensional separation medium (6). The inventive method comprises the following steps: the separation medium (6) is contained in a first space (2) extending substantially over all three spatial coordinates; a plurality of individual samples (29) is disposed in the vicinity of a boundary surface of the first space (2), whereby the individual samples (29) are disposed in such a way that they substantially constitute a surface i.e. the respective centres of gravity thereof are described by two coordinates; the individual samples (29) can migrate through the separation medium under the influence of one or several physical variables in a manner which is substantially perpendicular to the surface of application, whereby they are separated according to one or several properties thereof, said physical variables being, for example, electric forces (electrophoresis), gravitation, diffusion, pressure and concentration gradients and osmosis or centrifugal forces; the individual samples (29) are detected in selected areas (8) inside or outside the separation medium (6) during the migration thereof (online detection), 3D data detection upon completion of migration being an alternative to online detection, whereby a corresponding 3D image detection method can be used; in the simplest case, the separation medium (6) is decomposed into segments which are evaluated by means of a 2D detection method, whereby in this case the fractions of the samples are preparatively accessible; alternately or complementary to the image detection, a fraction collector can be arranged on a boundary surface of the separation medium (6).