METHODS OF SCREENING APOPTOSIS MODULATING COMPOUNDS, COMPOUNDS IDENTIFIED BY SAID METHODS AND USE OF SAID COMPOUNDS AS THERAPEUTIC AGENTS

摘要

The invention relates to a method of screening cellular polypeptides for pro-apoptotic or anti-apoptotic activity in a cell of a particular cell-type, said method comprising a. culturing cells of said particular cell-type under non apoptotic conditions and culturing cells of said particular cell-type under apoptotic conditions; and, b. determining subcellular localisation of said cellular polypeptides in the cultured cells; wherein a localization of a cellular polypeptide in lipid rafts in cultured cells under non apoptotic conditions and a segregation of said cellular polypeptide from lipid rafts in cultured cells under apoptotic conditions is indicative that said cellular polypeptide has a pro-apoptotic or an anti-apoptotic activity in said particular cell-type.