DENDRITICALLY AMPLIFIED DETECTION METHOD

摘要

A method and system for the detection of a target nucleic acid in a sample solution. The target nucleic acid comprises a first and a second end sequence, one of the end sequences being a 5' end sequence and the other end sequence being a 3' end sequence. The method comprises: (a) attaching to a solid surface a first oligonucleotide probe, at least a portion of which is complementary to the first end sequence of the target nucleic acid; (b) contacting the solid surface with the sample solution, thereby allowing the first probe to bind the target nucleic acid; (c) providing a second semiconductor nanoparticle to which has been attached a second oligonucleotide probe, at least a portion of which is complementary to the second end sequence of the target nucleic acid; (d) contacting the solid surface of step (b) with the second nanoparticle, thereby allowing the second probe to bind the bound target nucleic acid; (e) providing a first semiconductor nanoparticle to which has been attached the first oligonucleotide probe and pre-incubating the first nanoparticle with the target nucleic acid, thereby allowing the first probe to bind the target nucleic acid; (f) contacting the solid surface of step (d) with the pre-incubated first nanoparticle, thereby allowing the target nucleic acid bound to the first probe to bind the second probe on the second nanoparticle; and (g) detecting the presence of the nanoparticles on the solid surface, thereby detecting the target nucleic acid.