| 摘要 |
Embodiments of the invention generally provide methods and compositions for producing polyvalent antigens, disease-specific antigens, transglutaminase-reactive compounds, and recombinant transglutaminases. A method of producing a cross-linked compound by a biological agent is also provided. The invention provides a method for producing a cross-linked antigen and a method of using cross-linked products as antigens to immunize animals and induce strong immune responses. In addition, a method of producing an antigen specific for Alzheimer's disease is provided. Further, a method of producing a polyvalent antigen for two or more diseases is provided. Thus, compositions of antigens are prepared and provided to immunize animals and induce strong immune responses. The invention provides purified recombinant transglutaminases reactive to a broad range of compounds and exhibit broad substrate activity. Additionally, the invention provides a method of attaching one or more amino acid residues to a compound to be reactive with transglutaminase, even for transglutaminase non-reactive compounds. |
