Device for electrochemical detection of nucleotide sequences, analysis-cassette module specially adapted to the device and process of manufacture thereof

摘要

Apparatus for electrochemical detection of specific nucleotide sequences in a liquid (F) comprises: (a) analysis cassette (29) containing electrode coated with a biopolymer with specific affinity for the sequence and a counter electrode; (b) laptop computer (5) which can be connected to the cassette and a battery (9) and which processes signals produced by electrodes when a specimen is analyzed; and (c) thermostat for the cassette operated by the battery. Apparatus for electrochemical detection of specific nucleotide sequences in a liquid (F) comprises: (a) an analysis cassette (29) containing an electrode coated with a biopolymer with specific affinity for the sequence and a counter electrode; (b) a laptop computer (5) which can be connected to the cassette and has its own battery (9) and which processes signals produced by the electrodes when a specimen is analyzed; and (c) a thermostat for the cassette which is operated by the battery. Independent claims are included for: (1) the analysis cassette; (2) a set of analysis cassettes in which the electrodes are suitable for detecting different nucleotide sequences; (3) a module (19) for connection to a digital interface (33) on a computer which has an analog interface (35) for connection to the analysis cassette and a thermostat for controlling the temperature in the cassette; and (4) making the analysis cassette by fastening the working electrodes to a plastic molding, immersing them in biopolymer solution, attaching the counter-electrodes, folding the molding so that the electrodes are opposite each other and inserting the product into a case.