| 摘要 |
A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first an d second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplico n of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.
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