Nucleic acid assembly, vector, cell, methods and kit thereof

摘要

The present disclosure relates to a nucleic acid assembly (NAA), comprising sensor domain and handle domain; an assembly interfaceable motif (AIM) sequence optionally along with intracellular targeting motif (ITM) sequence; and an AIM-NAA complex. It also relates to a vector comprising assembly interfaceable motif sequence optionally along with intracellular targeting motif sequence and a cell comprising the vector. Further, the instant disclosure also provides a method to obtain the nucleic acid assembly, method of intracellular targeting and kit thereof.

主权项

1. A nucleic acid assembly (NAA) comprising sensor domain and handle domain, optionally along with sensor molecule; wherein the nucleic acid assembly is a duplex selected from the group consisting of SEQ ID Nos. 1 and 4, SEQ ID Nos. 2 and 4, SEQ ID Nos. 3 and 4, SEQ ID Nos. 5 and 6, SEQ ID Nos. 7 and 8, SEQ ID Nos. 9 and 12, SEQ ID Nos. 10 and 12, and SEQ ID No.s 11 and 12.