METHOD FOR TARGET DNA ENRICHMENT USING CRISPR SYSTEM

摘要

The present invention relates to a method of capturing a target nucleic acid sequence in genome sequencing, e using a CRISPR system. According to the present invention, the use of a plurality of CRIPSR systems enables capturing a plurality of target nucleic acids within genome simultaneously.

主权项

1. A method of capturing a target nucleic acid sequence in genome sequencing, the method comprising:
treating a genome sample including a target nucleic acid sequence, with a plurality of CRISPR systems that can cut at both ends of the target nucleic acid sequence or can complementarily bind to CRISPR complex-binding sequence within the target nucleic acid sequence, and sorting the target nucleic acid sequences from fragments of genome sample or PCR amplification products thereof, wherein one or more target nucleic acid sequences within genome are captured simultaneously.