| 摘要 |
<p>The present invention concerns a method and a kit for determining the presence or absence of at least one sequence variant in one or more target nucleic acids in an individual sample containing deoxynucleoside triphosphates, an agent for the template-dependent polymerization of the deoxynucleoside triphosphates, for example a DNA polymerase, at least one discriminating primer containing at least one discriminating nucleotide residue, wherein one primer is added for each sequence variant of a target nucleic acid to be detected, said primer having a sequence which is complementary to the sequence variant to be detected, and wherein the sequence variant to be detected in the target nucleic acid is complementary to at least one 3'-terminal, 3' proxi-terminal or 3' proxi-proxi-terminal nucleotide residue of the discriminating primer, characterized in that invention one or more nucleotide residues of at least one discriminating primer are substituted at the 4' position of the (deoxy)ribose, at least one additional primer which is complementary to a primer extension product that is formed by extension of a discriminating primer.</p> |
