| 摘要 |
The subject of the invention is a method for the identification and isolation of genome fragments with coupling disequilibrium, wherein regions of the genome are isolated, which contain candidate gene regions that are found in coupling disequilibrium with their constricted DNA environment, and these genome regions are obtained from individuals who are not related to one another as well as individuals who are related to one another. The earlier conducting of the cloning step, in comparison to other methods, makes possible an obtaining of DNA fragments that is independent of quantity and in addition replaces the methylation step conducted in other methods. It was also found that the use of a plant enzyme according to the invention brings about a much higher specificity of the method when compared with those in which the Mut S, H, L complex is used.
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