METHOD FOR ENHANCED UPTAKE OF VIRAL VECTORS IN THE MYOCARDIUM

摘要

The present invention relates to improved therapies for the treatment of heart disease, particularly the improved delivery of therapeutic agents to heart tissue by direct infusion into the coronary circulation. A preferred embodiment of the invention is a method of treating or preventing a cardiovascular disease by transfecting cardiac cells of a large mammal, the method comprising, identifying a mammal in need of treatment or prevention of heart disease, supplying NO to the coronary circulation prior to, and/or during the infusion of a therapeutic polynucleotide into a blood vessel of the coronary circulation in vivo, where the therapeutic polynucleotide is infused into the blood vessel over a period of at least about three minutes, where the coronary circulation is not isolated or substantially isolated from the systemic circulation of the mammal; and where the therapeutic polynucleotide transfects cardiac cells of the animal resulting in the treatment or prevention of the heart disease.

主权项

1. A method of treating or preventing a cardiovascular disease by transfecting cardiac cells of a large mammal, the method comprising:
identifying a mammal in need of treatment or prevention of a cardiovascular disease; administering a vasodilating substance to said mammal sufficient to dilate a blood vessel of the coronary circulation; and administering a therapeutic polynucleotide into a blood vessel of the coronary circulation in vivo; wherein said therapeutic polynucleotide is infused into said blood vessel over a period of at least about three minutes, wherein the coronary circulation is not isolated or substantially isolated from the systemic circulation of the mammal, and wherein said therapeutic polynucleotide transfects cardiac cells of said mammal resulting in the treatment or prevention of said cardiovascular disease.